Figure 1. G-CSF-induced HSC mobilization requires signals from the muscarinic receptor type-1 (Chrm1).

(A) HSCs/mL following G-CSF mobilization in wild-type mice treated with saline or Scopolamine hydrobromide (Scop 1mg/kg; n = 5–8). HSCs defined as Lineage⁻ Sca1⁺ cKit⁺ Flt3⁻.

(B) Schematic of G-CSF-induced mobilization and analyses. Wild-type mice are denoted by black bars and Chrm1^−/− mice by white bars.

(C) HSCs/ml of peripheral blood (Lineage⁻ Sca1⁺ cKit⁺ Flt3⁻; n = 12–16)

(D) Colony-forming units/ml of peripheral blood determined in vitro (CFU-C; n = 6–13).

(E) Lineage⁻ Sca1⁺ cKit⁺ (LSK) cells/femur at steady state (n = 9).

(F) Reconstitution after competitive BM transplantation of mobilized blood into irradiated hosts

(G) Tri-lineage engraftment of donor cells 16 weeks post transplantation: B220⁺ cells (B) CD4/CD8⁺ cells (T) and Mac1⁺ cells (M; n= 6–7).

* p < 0.05, ** p < 0.01 determined by Student’s t test. Data are represented as mean ± SEM. See also Figure S1.