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. 2017 Jun 12;12(6):e0179184. doi: 10.1371/journal.pone.0179184

Fig 6. Inhibition of c-Maf by PGE2 is independent of IL-21.

Fig 6

(A) Naïve CD4+CD62L+ cells were stimulated in the presence of IL-27 and PGE2 or dbcAMP. Supernatant was collected on day 3 and analyzed by ELISA for IL-21. Data are cumulative from two independent experiments. (B) Naïve CD4+CD62L+ cells from il10gfp mice were stimulated in the presence of IL-27, 100 ng/ml IL-21 and PGE2. Cells were collected on day 3 and analyzed by FACS to determine IL-10 production within CD49b+LAG-3+ CD4 T cells. Data are representative of two independent experiments. (C) Naïve CD4+CD62L+ cells from C57BL/6 mice were stimulated as above. Cells were collected on day 3 and analyzed by FACS to determine presence of intracellular c-Maf within CD4 T cells (top) and CD49b+LAG-3+ CD4 T cells (bottom). Histograms show representative samples, while graphs present data from one of three independent experiments. Each sample was tested in duplicate and results represent means ± SD. Significance was determine by one-way ANOVA; **P<0.01, ***P<0.001.