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. 2017 Jun 12;12(6):e0179184. doi: 10.1371/journal.pone.0179184

Fig 8. PGE2 inhibits Tr1 differentiation in vivo.

Fig 8

(A) Schematic of timeline and experimental groups. Il10gfp mice were injected i.p. with vehicle, 20 μg/ml anti-CD3 or anti-CD3 and 4μg dmPGE2 at 0hr and 48hr. Peyer’s patches, spleen, and intraepithelial lymphocyte populations were collected. (B) CD4+ populations were analyzed by FACS for Tr1 cell populations (CD49b+LAG-3+). (C-D) CD4+ populations were analyzed by FACS for IL-10 expression in both Tr1 populations and non-Tr1 populations (including CD49b+LAG-3-, CD49b-LAG-3+ and CD49b-LAG-3- populations). Data are cumulative of two independent experiments. Significance was evaluated by unpaired t-test.