Figure 4.

AZA promotes Treg suppressive activity. (A, B) Representative histograms (A) and comparative panels (B) of the suppression of CD4⁺CD25⁺CD127^low Treg sorted from spleen of transplanted NSG mice, treated or not with AZA 5 mg/kg, on day 28. Treg were added to autologous CD3/CD28-stimulated Tconvs at a ratio of either 1:1, 1:4 or 1:16 (Treg:Tconv) and proliferation was measured by CFSE dilution, in triplicates. Dotted curve: Tconv alone; tinted curve: Tconv+Treg. Data show mean values of three wells/condition with SEM.