Figure 6.

AZA induces long-term tolerance. Spleen from NSG mice transplanted with 2 × 10⁷ human PBMC and receiving or not AZA 5 mg/kg were harvested on day 28, were homogenized and pooled (CTRL with CTRL and AZA with AZA). Spleen homogenates were either infused per se to 2.5Gy-irradiated NSG mice (4 × 10⁶ _huCD45⁺ cells/mouse), or were depleted of human CD25⁺ cells before infusion (4 × 10⁶ _huCD45⁺ cells/mouse). (A, B) Survival and GVHD scoring curves (n = 6/condition) of mice transplanted with total spleen homogenates. (C) FACS comparison of CD25⁺FOXP3⁺ cell frequency within the CD4⁺ T cell population in blood at 14 and 40 d post-transplantation. (D) Survival curve (n = 6–7/condition) of mice transplanted with either total or CD25⁺-depleted spleen homogenates. (E) FACS comparison of CD25⁺FOXP3⁺ cell frequency within the CD4⁺ T cell population in blood at 14 d post-transplantation. Data show median values of 6–7 mice/condition with interquartile range (*p < 0.05, **p < 0.005, ***p < 0.0005).