Figure 4.

Phagocytosis of sHS-treated tumor cells partially depends on calreticulin and leads to a phenotypic maturation of DC in vitro. (A, B) Untreated cells or cells treated with mHS (42°C, 1 h) or sHS (47°C, 1 h) followed by incubation at 37°C for 24 h were incubated with DC for additional 24 h. Graphs show percentage of DAPI negative DiO⁺DiD⁺ DCs and represent means ± SEM of five donors or four mice (*p < 0.05). (C) The expression of maturation markers on DCs after 24 h in cultures where DC + HS-treated tumor cells were co-incubated or where HS-treated cells were separated from DCs by a transwell. (D) IL-10, IL-12p70 and TNF-α production was determined from cell culture supernatants by ELISA after 24 h. The graphs are means ± SEM of 6–8 donors (*p < 0.05).