Figure 5.

DCs pulsed with sHS-treated tumor cells stimulated antigen-specific CD4⁺ T cells and induced higher CD8⁺ T cell activation and proliferation. DCs incubated with sHS or mHS-treated cells for 24 h were added to autologous T lymphocytes at ratio 1:10. The number of IFNγ producing (A) CD8⁺ and (D) CD4⁺ T cells, proliferation (Ki67) and activation (CD137) of (C) CD8⁺ and (D) CD4⁺ T cells was determined after 7 d after one round of restimulation. (B) The number of MP1_58–66-specific CD8⁺ T cells was determined after 9 d. (E) Percentage of Foxp3⁺CD127^low T regulatory cells from CD4⁺CD25⁺ T cells. Dotplots are representative of 4–8 donors. Graph shows percentage of IFNγ⁺CD8⁺ T cells or IFNγ⁺CD4⁺ T cells from CD8⁺ or CD4⁺ T cells, respectively and represents means ± SEM of 4–8 donors (*p < 0.05). (F) Immunoblot of tumor antigens after HS treatment. Immunoblots are representative of n = 3.