Figure 6.

sHS-treated tumor cells are immunogenic and elicit prophylactic immunity in mice. (A, B) The viability of untreated CT26 cells and cells treated with mHS (42°C, 1 h) or sHS (47°C, 1 h) followed by an incubation at 37°C for 5 h and 24 h. (C) The exposure of HSP70, HSP90 and calreticulin was determined by flow cytometry from AnnexinV+DAPI− cells. Graphs represent means ± SEM of n = 3 (*p < 0.05). Dotplots are representative of n = 3. (D) Mice were vaccinated with sHS or mitoxantrone-treated CT26 cells at days 0 and 21 and challenged with live CT26 at day 31. Tumor growth and survival was monitored in 10 mice per group. Three out of 10 mice were considered long-term survivors after day 100. The results are representative of n = 3. (E) Colony-forming assays were assessed after 14 d.