Figure 4.

Mice reconstituted with γδT17 cells have milder colitis. (A and B) Neonatal thymocytes from WT mice were transferred into lethally irradiated TCR δKO mice following BM cell transfer from TCR δ KO mice. Mice were reconstituted for at least eight weeks. Mice with BM cell transfer alone were used as control. γδT cell and intracellular IL-17 and IFNγ staining was performed in mLN (A) and LPL (B), respectively. (C) Reconstituted mice were fed with DSS water for 7 d. Representative histological slides and accumulative scores are shown. (D) Representative dot plots of Gr-1 and CD11b staining in LPL are shown. (E) Splenocytes from OT-1 mice were labeled with CFSE and then co-cultured with γδ T cells sorted from LPL of DSS-treated WT mice (day 7) in the presence of OVA for 3 d. Cells were stimulated with PMA+ionomycin and intracellular IFNγ staining was performed. Cells were gated on CD8⁺ cells. Representative dot plots and summarized IFNγ-producing CD8 T cells are shown. (F) LPLs from DSS-treated mice were stained with CD3, CD4, γδ TCR, and intracellular galectin-1 and galectin-9. Summarized percentages of galctin-1 or galectin-9-positive CD4 or γδ T cells are shown. *p < 0.05, **p < 0.01.