Figure 3.

Transferred HLA-A*02:01/CHM1³¹⁹ T cell receptor transgenic T cells home to bone marrow of patient #2 and persist. (A) Bone marrow (BM) aspirates from the left posterior iliacal spine (LPIS) stain positive for the CHM1³¹⁹ multimer at a stronger rate than BM of the right posterior iliacal spine (RPIS) at day 6 after first adoptive transfer. Peripheral blood (PB) cells do not stain CHM1³¹⁹ multimer positive. (B) At day 14 after first transfer BM aspirates and peripheral blood cells convert CHM1 multimer negative, demonstrating loss of adoptively transferred T cells after first adoptive transfer (x-axis; CHM1³¹⁶multimer⁺/irrelevant multimer⁺ CD8⁺ staining ratio). (C) and (D) At day 14 after second adoptive transfer BM aspirates show a stronger CHM1³¹⁹ multimer flourescence in RPIS but CHM³¹⁹ loss in LPIS. PB is CHM1³¹⁹ multimer positive and HLA-A*02:01/CHM1³¹⁹ T cell receptor transgenic T cells proliferate until last PB draw on day 27 after second (day 56 after first) adoptive transfer. An irrelevant EZH2⁶⁶⁶/HLA-A*02:01 restricted multimer serves as control (CD8⁺ gate).