Figure 1.

Western blotting analysis. (1A and 1B): Reactivity of serial serum samples from two representative LC patients in group 1 to LC cell line H1299. Patient 1 was a 69-y-old Caucasian male who was a current heavy smoker (more than 50 pack years) and started to smoke at the age of 16-y-old. He was drawn sequential serum samples between year of 2003 and 2011 and diagnosed as lung adenocarcinoma of stage IA in Oct. 2009. As shown in 1A, in the initial serum sample collected in May 2003, autoantibodies were barely observed, but appeared one year later, 5 y before the diagnosis of LC. The 34 kD band (arrow) arose at the time point of 1.5 y before the lung cancer diagnosis (lane 5 in 1A) and reached at the highest titer at the time of 3 mo before the diagnosis (lane 6 in 1A). Fig. 1(B) Shows a 64-y-old Hispanic male, who has been smoking for 44 y with average 32 pack years, since he was 20 y old and diagnosed as small cell LC in stage IA when he was 64-y-old. A 34 kD reactive band (arrow) was observed in the initial serum sample collected in Sept. 2003 (lane 1 in 1B), but the titer was sharply increased 4 y later that was the time point of 3 y (lane 4 in 1B) before LC diagnosis. (1C) Reactivity of serum samples from five lung cancer patients in group 1, which drawn at the diagnosis of LC and five normal individuals in group 2 to H1299 cell line. 1D-1I: Immunoreaction of recombination protein ECH1 (1D–1F) and HNRNPA2B1 (1G–1I) to the serial serum samples from two representative patients the same with 1A and 1B, and to 10 representative individual sera (1F and 1I) from LC patients in group 3. *Time of lung cancer diagnosis (patient 1 was diagnosed in Oct. 2009 and patient 2 in April 2001).