Figure 2.

Immunoreactive proteins were detected by autoantibodies in serum samples from lung cancer patients. 2-DE gel electrophoresis of H1299 subcellular fractions (cytosolic, membrane and nucleus) were stained by Coomassie blue (2A, 2D and 2G). 2-DE Western blotting of H1299 subcellular fractions with the pool of sera from LC patients (n = 5 from group 1: line 1–5 in Fig. 1C) (2B, 2E and 2H) and with the pool of sera form normal individuals (n = 5 from group 2: line 6–10 in Fig. 1C) (2C, 2F and 2I).