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. 2017 Apr 20;6(5):e1312042. doi: 10.1080/2162402X.2017.1312042

Figure 1.

Figure 1.

TFEB is involved in the regulation of macrophage M2 polarization. (A) MΦs were isolated from tumor tissues of mice bearing 4T1 (n = 8) or EO771 (n = 8) tumors. TFEB mRNA expression in macrophages was determined by qPCR. *p < 0.05 vs. pMΦ-naive, two-tailed Student's t-test. (B) Peritoneal macrophages were cultured with or without EO771 or LLC tumor-conditioned medium (TCM) for 24 h. *p < 0.05 vs. control; two-tailed Student's t-test. (C) Western blot analysis of TFEB protein in mouse peritoneal MΦs treated as in (B). (D) TFEB expression in mouse peritoneal MΦs transduced with control shRNA lentiviruses (LV-Con) or two different TFEB shRNA lentiviruses (LV-TFEB-sh2 or LV-TFEB-sh3). *p < 0.05 vs. LV-Con; two-tailed Student's t-test. (E) Western blot analysis of TFEB in peritoneal MΦs treated as in (D). (F) Effects of TFEB knockdown using LV-TFEB-sh2 in peritoneal MΦs on the expression of M2 markers Arg-1 and YM-1 and M1 markers iNOS and TNF-α. Peritoneal MΦs were treated with IL-4 (15 ng/mL) or TCM, or LPS (10 ng/mL) for 24 h. *p < 0.05 vs. LV-Con under the same treatment; one-way ANOVA followed by the post-hoc Dunnett's test.