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. 2017 Apr 20;6(5):e1312042. doi: 10.1080/2162402X.2017.1312042

Figure 5.

Figure 5.

SOCS3-STAT3 pathway plays a key role in TFEB silencing-enhanced macrophage M2 polarization. (A) MΦs were transduced with control or TFEB shRNA lentiviruses and treated with IL-4 (15 ng/mL) or EO771 TCM for 24 h. The expression of SOCS3 was analyzed by qPCR. *p < 0.05 vs. corresponding LV-Con group. (B) Cellular proteins were analyzed by Western blot analysis after the cells were treated as in (A). (C) Cellular proteins were analyzed by Western blot analysis after MΦs were treated as in (A), with or without pretreatment with stattic (5 μM) for 2 h. (D) MΦs were treated as in (C). The expression of Arg1 and YM-1 mRNA was analyzed by qPCR. *p < 0.05 vs. corresponding control.