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. 2005 Feb;71(2):883–892. doi: 10.1128/AEM.71.2.883-892.2005

FIG. 2.

FIG. 2.

Uracil auxotrophy and FOA resistance conferred by pyrF deletion is complemented by plasmid-borne pyrF. (A) Strains P. putida KT2442 (squares) and P. putida TEC1 (circles) were grown at 30°C in liquid M9 minimal medium supplemented with citrate in the presence (solid symbols) or absence (open symbols) of uracil at 20 μg/ml. Growth was monitored at 600 nm after inoculation to 0.05 at 0 h. (B) Strains P. putida TEC1 carrying pKT231 (circles) or pKP (pKT231 carrying pyrF under its own promoter) (squares) were grown as in A. (C) Upper panel: Deletion of pyrF confers uracil auxotrophy and FOA resistance. Serial dilutions of P. putida KT2442 or TEC1 were plated on solid M9 minimal medium in the presence or absence of uracil (20 μg/ml) or with uracil and 5-fluoroorotic acid (250 μg/ml). Lower panel: Serial dilutions of P. putida TEC1/pKP were plated as above but with kanamycin, the resistance marker of pKP.