Strains |
|
|
P. putida KT2442 |
Rifr; derived from P. putida KT2440 |
15 |
P. putida TEC1 |
Rifr; P. putida KT2442 with a nearly complete internal deletion of pyrF
|
This work |
P. putida TEC1 ΔbetCDE
|
Rifr Gmr; TEC1 whose betCDE putative operon was deleted by use of pTGLB |
This work |
E. coli CC118λpir
|
Δ(ara-leu) araD ΔlacX74 galE galK phoA20 thi-1 rpsE rpoB argE recA1 λpir lysogen |
15 |
E. coli HB101 |
Smr; rpsL recA thi pro leu hsdR−M+ (E. coli K-12/E. coli B. hybrid) |
37 |
E. coli X82 |
lacZ53(Am) λ−trpC50 pyrF287 hisG1(Fs) rpsL8; uracil auxotroph |
E. coli Stock Center CGSC 5889 ID 6952 |
Plasmids |
|
|
pUC19NotI |
Apr; pUC-type plasmid with MCS of pUC19 flanked by NotI sites |
15 |
pUP |
Apr; pUC19NotI inserted with a 2-kb EcoRI-HindIII PCR fragment containing the P. putida pyrF chromosome region |
This work |
pUPX |
Apr; pUP with an xylE SmaI fragment from pXYLE10 cloned into the Eco47III and SmaI sites of pUP |
This work |
pKNG101 |
Smr; sacRB+, broad-host-range plasmid of conditional replication |
17 |
pKPX |
Smr; pKNG1 with the pUPX ΔpyrF::xyIE NotI fragment |
This work |
pKT231 |
Kmr, Smr, broad-host-range plasmid derived from RSF1010 |
10 |
pKP |
Kmr; pKT231 with the P. putida pyrF gene and its promoter region |
This work |
pGAPN |
Apr; pGEM-T Easy carrying a PCR fragment of pyrF (with endogenous promoter) |
This work |
pGP704 |
Apr; oriR6K, mobRK2, MCS of M13 tg131 |
25 |
pGEM-T Easy |
Apr; PCR fragment cloning plasmid |
Promega |
pGGLB |
Apr; pGEM-T Easy with the betCDE deletion cassette cloned into the NotI site |
Galväo et al., unpublished |
pTEC |
Kmr FOAs Ura+; ligation of MCS-Km-MCS, oriR6K/origin of transfer mobRK2, and pyrF PCR fragments |
This work |
pTGLB |
Kmr Gmr FOAs Ura+; pTEC with the betCDE deletion cassette cloned into the NotI site |
This work |
pRK600 |
Cmr; oriColE1, mobRK2, traRK2 |
19 |