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. 2017 Jun 12;7:3328. doi: 10.1038/s41598-017-02969-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of microRNA. (A–F) Boxplots showing the distribution of normalised Ct following Taqman individual microRNA assays of miR-19b-3p, miR-21-5p, miR-204-5p, miR-223-3p, miR-451a and miR-886-3p in each of the four sets of samples: CH (N = 25), CND (N = 25), SE (N = 16) and TLE (N = 14). Note, the data shown are normalized and the lower the value the greater the relative expression of the microRNA. Outliers not graphed for purposes of data presentation are shown as circles. (G) log₂(FC) and adjusted p-value for validated microRNA. CH: controls with headache, TLE: temporal lobe epilepsy, SE: status epilepticus, CND: controls with other neurological diseases.