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. 2017 Jun 12;7:3289. doi: 10.1038/s41598-017-03315-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Cell concentrations of the enrichment cultures calculated from qPCR data of A_DKE, B_DKE and C_DKE, (b) with corresponding pH values and Fe(II) concentrations over a time period of 11 weeks. Corresponding CARD-FISH pictures of enrichment cultures showing: (c) co-culture after four weeks of growth (B_DKE and C_DKE stained in red (ARCH915, Alexa 546) and A_DKE stained in green (ARM980, Alexa 488)) and (d) after eight weeks of growth (again B_DKE and C_DKE stained in red (ARCH915, Alexa 546) and A_DKE stained in green (ARM980, Alexa 488)).