TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Escherichia coli DH5α | recA1 endA1 gyrA96 thi relA1 hsdR17 (rk− mk+) supE44 Φ80dlacZΔm15 ΔlacU169 | 6 |
| Bacillus subtilis 168 | trpC2 | Laboratory stock |
| Bacillus subtilis 168 ΔRA | trpC2 ΔxylRA | Laboratory stock |
| WH476 | trpC2 ΔxylRA amyE (control region and spoVG-lacZ from pWH102, Cmr) | This study |
| WH478 | trpC2 ΔxylRA amyE (control region and spoVG-lacZ from pWH105, Cmr) | This study |
| pWH1411-BD | Cmr, tetR(BD) | 25 |
| pWH1520 | Apr Tcr, xylR xylA′ | 22 |
| pWH353 | Kmr Apr, improved Pr* promoter upstream of tetR(B) | 4 |
| pDH32M | Apr Cmr, spoVG-lacZ | 9 |
| pHT304 | Apr Err, pUC19 polylinker | 1 |
| pWH102 | Apr Cmr, 68-bp control region with tetO bearing EcoRI-HindIII fragment upstream of spoVG-lacZ | This study |
| pWH105 | pWH102, second tetO cloned in BamHI site | This study |
| pWH1520-BD | pWH1520 with a 668-bp tetR(BD)-containing HincII fragment from pWH1411-BD cloned in SmaI site | This study |
| pWH116-BD | pHT304 with a xylR′-xylA′-tetR(BD)-bearing 1,453-bp EcoRI-SphI fragment from pWH1520-BD | This study |
| pWH117-BD | pHT304 with Pr* from pWH353 cloned via EcoRI-ApaI | This study |
| pWH118-BD | pHT304 with spoVG promoter from B. subtilis 168 cloned via PCR and EcoRI-XbaI | This study |
| pWH119-BD | pHT304 with xylA promoter from B. subtilis 168 cloned via PCR and EcoRI-XbaI | This study |
| pWH123-127 | pHT304 with synthetic promoter cloned via EcoRI-XbaI (see Fig. 4C) | This study |