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. 2017 Jun 12;7:3296. doi: 10.1038/s41598-017-03740-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DC-SIGN is expressed in the macrophages of plaques and the macrophages of atherosclerotic patients. Human femoral arteries from patients with angiographic atherosclerotic plaques and internal thoracic arteries without plaques were assessed by histological and immunochemical analysis. As a continuity study, the origin of tissues is same as our previous study²². Sections were stained with hematoxylin and eosin or immunofluorescence stains for DC-SIGN, TLR4 and CD68.