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. 2017 Jun 12;7:3221. doi: 10.1038/s41598-017-02748-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of NBO treatment on ischemic brain edema formation. (A) Brain section stained with TTC from animals that studied in experimental groups. Sever edema caused noticeable swelling and deformation of the left hemisphere in control and NBO-treated animals. (B) Brain edema formation in experimental groups. (C) Brain water content in the left and right sides of the brains in the studied groups (n = 7–8). Values are mean ± SEM. (***P < 0.001, compared with sham group; ^† P < 0.001, compared with right side of the same group).