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. 2017 Jun 12;7:3323. doi: 10.1038/s41598-017-02532-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Spreading and proliferation of OD21 cells in 3D GelMA hydrogels. Representative images of OD21 cells encapsulated in (A) 5%, (B) 10% and (C) 15% GelMA hydrogels stained for actin (green) and DAPI (blue) shows increased cell spreading in stiffer over softer hydrogels. (D) Quantification of the number of cells per gel after 1, 4 and 7 days in culture indicates a significantly higher rate of proliferation of OD21 cells in scaffolds of higher polymer concentration. Statistical significance is represented by *for p < 0.05, ***for p < 0.001 and ****for p < 0.0001.