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. 2017 Jun 12;7:3215. doi: 10.1038/s41598-017-03033-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Clay distribution in ATPS having reverse volume ratio. For 20%/20% w/w PEG 8 kDa-Dx 10 kDa using volume ratio of 1:20 PEG-rich phase: Dx-rich phase with (A) Kaolinite (KGa-2), (B) Na-rich montmorillonite (SWy-2), (C) Ca-rich montmorillonite (STx-1b) and (D) NX-illite. Labeled Dx (red, 2 μM final concentration) and labeled PEG (green, 2 μM final concentration) have been used for fluorescence imaging. Left-hand images show transmitted light, and right-hand images show overlay of red and green confocal fluorescence channels. Continuous phase is Dx-rich phase while droplets are PEG-rich phase.