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. 2017 Jun 12;7:3321. doi: 10.1038/s41598-017-03215-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Map of expression vector pHT43-Y106-EG (sub-cloning the alkali-tolerant Y106-eg in a shuttle vector pHT43), (b) Proteins detected by SDS-PAGE. Y106-WT: Crude enzyme of Bacillus subtilis Y106; Y106OE-EG-1/Y106OE-EG-2: Crude enzyme of Bacillus subtilis Y106OE-EG; Purified EG: EG purified from crude enzyme of Bacillus subtilis Y106OE-EG, M: protein molecular weight markers (kDa).