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. 2017 Jun 12;7:3238. doi: 10.1038/s41598-017-03549-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal micrographs showing localization of the α1, β1, δ, ε, and γ subunits at the NMJs and muscle membrane of (A) DIV (grade IV) facial nerve injury of the orbicularis oris on day 7 and (B) DIV facial nerve injury of the orbicularis oris on day 30. Immunofluorescence staining with α-bungarotoxin (green, mark α1 subunit), β1, or δ or ε or γ subunits (pink), and neurofilament 200 (red, mark nerve). Large arrows mark the NMJ, and small arrows mark the muscle membrane. Scale bars = 30 μm.