Figure 1.

In silico evaluation of target specificity for the annealing blockers of Anopheles and Mammalia. The y-axis represents the relative fraction of 18s rRNA gene sequences present in version 119 of the Silva database that match the primer indicated on the x axis, with match defined as a weighted score <1.0 (See Methods). (A) Specificity for V9 region annealing blockers. (B) As in A, but for the V4 region annealing blockers. As positive controls, the reference universal eukaryotic PCR amplification primers V9-Forward and V4-Reverse primers were also queried against the Silva database (right bar in each frame).