Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 12;7:3241. doi: 10.1038/s41598-017-03487-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Identification of PNA oligonucleotide blocker candidates for the V9 region of Anopheles. In order to design PNA blockers specific to Anopheles, but allowing efficient amplification of other eukaryotic organisms, the Silva eukaryotic database was queried with mosquito candidate sequences to identify those with the smallest number of non-mosquito hits. (A) The full-length sequence of the V9 hypervariable region of A. gambiae was split in silico into all possible 17mers, and each 17mer was queried against the full length 18s rRNA gene sequences from the Silva 119 database for all non-mosquito eukaryotes. The number of matches was graphed (y-axis), limiting the representation to a maximum of 100 hits. The location of the selected current PNA blockers is indicated (red vertical line). (B) As in A, but for V4 region PNA oligonucleotide candidates.