Figure 3.

Taxonomic profile of 18s rRNA gene amplicons for the V4 hypervariable region generated under different blocking conditions. Mammalian and mosquito hits are shown in grey scale while those of eukaryotic microbes are shown in colour. In all three samples, use of the mosquito specific PNA blocker significantly decreased mosquito reads. In animal and human bloodmeal samples with PNA blockers, human and mammal reads were predominant, whereas in the larval pool sample, eukaryotic microbes represented the majority of sequence reads when PNA blockers were used. For simplicity, sequences were collapsed at level 6 of the Silva 119 eukaryotic taxonomy, and lineages with less than 100 total amplicons were collapsed into the Other class (12 lineages for the V4 hypervariable region; 23 lineages for the V4 hypervariable region) to facilitate legend visualization.