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. 2017 Jun 12;7:3241. doi: 10.1038/s41598-017-03487-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Taxonomic profile of 18s rRNA gene amplicons for the V9 hypervariable region generated under different blocking conditions. Mammalian and mosquito hits are shown in grey scale while those of eukaryotic microbes are shown in colour. In all three samples, use of the mosquito specific PNA blocker decreased mosquito reads, though to a lesser extent than with the V4 PNA blocker (compare to Fig. 3). Sequences were collapsed at the V6 level of Silva 119 eukaryotic taxonomy, and lineages with less than 100 total amplicons were collapsed into the Other class to facilitate legend visualization.