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. 2017 Jun 12;7:3222. doi: 10.1038/s41598-017-03397-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TUNEL labeling to measure cell death 8 weeks after sub-retinal injection. (a) Quantification of TUNEL positive cells in AAV1-BRI-Aβ vector injected eyes. (b–e) Representative images of TUNEL labeling to identify apoptotic cells at 400X magnification in (b) un-injected eye (c) AAV1-BRI-Aβ40 (d) AAV1-BRI-Aβ42 and (e) AAV1-BRI-Aβ40+AAV1-BRI-Aβ42 injected eyes. Scale bar: (b–e) = 20 μm. ONL: outer nuclear layer; INL: inner nuclear layer; IPL: inner plexiform layer; RGC: retinal ganglion cells. *p < 0.05 is considered significant, ***p < 0.001 is considered highly significant.