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. 2017 Jun 12;7:3304. doi: 10.1038/s41598-017-03508-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic tree of the Dicistroviridae ORF1 region. The tree includes the extended contig n1905 (proposed KBV strain) and LHUV-1 using a Le & Gascuel (LG)⁵⁴ model with 500 bootstrap replicates. The ORF1 sequences included in the analysis comprise the complete Helicase, Protease and Ribosomal dependent RNA polymerase (RdRp) domains of these viruses and intergenic regions. Macrobrachium rosenbergii Taihu virus and Mud crab discistrovirus-1 (two unclassified Dicistroviridae that had ~50% identity to our contigs in blastp results) were included to root the tree. Our sequences are shown in bold text. GenBank accession numbers are shown in brackets.