Table 2.

Prediction of Gram⁺ and Gram^- secretory proteins from their mature domains.

Classifier	AUC (%)
	Gram−	Gram+
Preprotein (#P1)	97.96	97.59
MatureP (#M22)	85.79	90.04
Disorder (#M7)	74.61	82.4055

We measured the performance of the main classifiers (“preprotein” and “mature domain”) on a set of secretory proteins predicted in other bacterial proteomes. For this analysis we selected 25 Gram^- and 10 Gram⁺ bacteria and collected 7120 and 1361 secretory proteins correspondingly (see Methods; Table S5). The E. coli K-12 secretome was excluded since it was used for the initial training.