Figure 1.

Laser capture-RNA-Seq workflow. (A) Schematic of steps that were optimized in the current study (LCM instrument, RNA extraction and RNA-Seq library kit). (B) Representative images of (left) nissl stained mouse hippocampus from Allen Brain Atlas, (middle) Amigo2-EGFP mouse hippocampus showing the fluorescently labeled CA2 cells and projections prior to LCM and (right) post LCM. (C) Representative image of a glass slide with five dehydrated mouse brain sections post-LCM of hippocampal subregions CA1 and CA2. On the left is a magnified image of the dashed box. Scale bars: 500 μm (B,C left) and 4 mm (C right). CA, cornus ammonis; DG, dentate gyrus; LCM, laser capture microdissection; RIN, RNA Integrity Number.