Figure 3.

Hesperidin does not affect astrocytes number and glial fibrillary acidic protein (GFAP) levels. Adult mice treated i.p with vehicle or 10 mg/kg of hesperidin were submitted to immunohistochemistry analysis for GFAP (A–C) in the hippocampal CA1 area, and western blotting analysis of the level of GFAP (G). Cultured cortical astrocytes were treated with hesperidin and analyzed by immunonocytochemistry (D–F) and western blotting for GFAP (G). The number of astrocytes and the levels of GFAP did not change between control and hesperidin groups in vivo and in vitro. Scale bar, 20 μm, respectively. n = 4–6 animals per experimental group and n = 3 independent astrocyte cultures. Student’s t test.