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. 2017 Jun 13;8:1042. doi: 10.3389/fmicb.2017.01042

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Copyright © 2017 Olins, Rogers, Preston, Ussler, Pargett, Jensen, Roman, Birch, Scholin, Haroon and Girguis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of selected functional genes across the whole dataset related to (A) carbon fixation, (B) nitrogen metabolisms, (C) methanogenesis, (D) sulfur oxidation, (E) other sulfur metabolisms, (F) iron, and (G) hydrogen. Of these genes, only ammonia monooxygenase showed statistically significant differences in expression between sampling methods (higher expression in Niskin samples). Genes shown in blue showed statistically significantly more expression in Sites 1–4, while those in yellow were statistically significantly more expressed in Site 4 samples.