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. 2017 Jun 13;8:1072. doi: 10.3389/fmicb.2017.01072

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Copyright © 2017 Heimerl, Flechsler, Pickl, Heinz, Salecker, Zweck, Wanner, Geimer, Samson, Bell, Huber, Wirth, Wurch, Podar and Rachel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Two examples of cylindrical macromolecular complexes underneath the OCM. (A,B) Showing details of single slices of the respective tomograms, arrow points to the ending of the cylindrical complex at the OCM; OCM, outer cellular membrane; CPP, cytoplasmic protrusion; bar 50 nm each; (C,D) combined view of the respective sub-volumes, showing segmented macromolecular complexes (blue and yellow) in a volume texture rendering display, additional filamentous structures are labeled in green; (E,F) show cross-sections of the subvolumes with “ObliqueSlice” tool; orthogonal to the xy plane an oblique plane was chosen that cuts through the structure “parallel” to the OCM; arrow points to the inner “ring” of the structure; (G,H) top view of segmented macromolecular complexes; note that the yellow and blue parts serve as a color coding for what is coming out of/going through the plane.