FIGURE 4.

Analyses of stomata closure and relative expression level of slow anion channel (SLAC)-associated genes. (A) Percentages of three levels of stomata (completely open, partially open, and completely closed stomata) in sapk2 mutants and wild type plants were calculated before and after drought stress (n = 98 stomata for S2–1, n = 95 stomata for S2–7, n = 98 stomata for wild type plants). (B) Stomata numbers per mm² in 10 leaves from sapk2 mutants and wild type plants, respectively, were counted to indicate stoma densities. (C) Transcript level of stomata genes OsSLAC1 and (D) OsSLAC7 in sapk2 mutants and wild type plants before and after drought stress. Error bars indicate the SD of three independent experiments. ^∗∗indicate statistically significant differences between mutant lines and wild type plants (P < 0.01).