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. 2017 Jun 13;8:1040. doi: 10.3389/fmicb.2017.01040

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Copyright © 2017 Grande, Celia, Mincione, Stringaro, Di Marzio, Colone, Di Marcantonio, Savino, Puca, Santoliquido, Locatelli, Muraro, Hall-Stoodley and Stoodley.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Physicochemical characterization of L. reuteri DSM 17938 through DLS and Nanosight NS300 system (NTA). The DLS histogram analysis of bMVs and pMVs are shown in (A,B) respectively. bMVs and pMVs had a bimodal distribution of nanovesicles. The nanoparticle tracking distribution of bMVs and pMVs is shown in (C,D). Figures are representative of ten independent replicates for both DLS and NTA analysis. The size distribution represents the wide distribution of vesicles. The size distribution was calculated as intensity (%) using the multimodal distribution of software (Malvern Instruments Ltd.). Arrows show the mean peaks of particles at 209 nm (Peak 1), 1446 nm (Peak 2), and 1921 nm (Peak 3) as herein reported in Sub-section “Physicochemical Characterization of pMVs and bMVs by DLS” of Section “Results.” The error bars represent the standard deviation.