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. 2017 Jun 13;5:35. doi: 10.3389/fbioe.2017.00035

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Copyright © 2017 Angelozzi, Penolazzi, Mazzitelli, Lambertini, Lolli, Piva and Nastruzzi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Production and characterization of composite microfibers. (A) Schematic representation of the snake micromixing microfluidic device employed for the production of composite microfibers. The device presents two inlets where the alginate suspension containing cells or gelatin or UBM were delivered through the micromixing snake geometry channel and a 700-µm outlet tube (#T3) into a BaCl₂ solution in order to obtain alginate (Af), alginate plus gelatin (AGf), or alginate plus UBM (AUBMf) microfibers. (B) Photomicrographs of Af, AGf, and AUBMf obtained at different pumping rates (from 0.5 to 1.5 mL/min). Arrows indicate the presence in AUBMf of UBM particles, in form of flat flakes. Bar corresponds to 500 µm. (C) Effect of the pumping rate on the dimension of the produced microfibers.