Figure 4.

Properties of the embedded cells: presence of secretory vesicles and metachromatic areas in the extracellular matrix (ECM). Af, AGf, and AUBMf embedded cells at days 7 and 14 of culture were stained with toluidine blue. Representative photomicrographs showed the presence of ECM deposition in the pericellular space of the single cells (as clearly evidenced in the higher magnification images) and the presence of metachromatic area (pink). Black arrows: single or UBM-attached cell. Bar corresponds to 50 µm for photomicrographs (A,D,G,J,M,P), to 25 µm for photomicrographs (B,E,H,K,N,Q), and to 10 µm for photomicrographs (C,F,I,L,O,R).