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. 2017 Jun 13;5:35. doi: 10.3389/fbioe.2017.00035

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Copyright © 2017 Angelozzi, Penolazzi, Mazzitelli, Lambertini, Lolli, Piva and Nastruzzi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chondrogenic properties of Af, AGf, and AUBMf embedded cells: transmission electronic microscope ultrastructural analysis at day 14 of culture. Lower magnification photomicrographs showed the ultrastructure of an embedded redifferentiated chondrocyte in Af (A), AGf (B), and AUBMf (C). In the images at higher magnification, red arrows indicated the presence of ECM dense material and collagen fibers with their typical banding pattern in the pericellular space (D,E) and evidenced the ECM-containing vesicles release from embedded cells (F,G). Bar corresponds to 2.6 µm in a-c, 1 µm in (D–F) and 0.4 µm in grams. C, cell; U, UBM.

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