Figure 1.

Pressure overload-induced cardiac fibrosis was alleviated in puerarin-treated mice. (a) Histological sections of the left ventricle in indicated groups were stained with PSR for fibrosis (upper and middle panel, scale bars: 100 μm). α-SMA was detected with immunohistochemistry (lower panel, scale bars: 200 μm). (b) TGF-β1/Smad2 signaling pathway and α-SMA protein in indicated groups were determined by WB, normalized to GAPDH (n = 6). ^∗p < 0.05 versus sham + vehicle group; ^#p < 0.05 versus TAC + vehicle group.