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. 2017 May 22;114(23):E4574–E4581. doi: 10.1073/pnas.1703057114

Fig. S3.

Fig. S3.

Vimentin regulates Jagged endocytosis, Notch receptor transendocytosis, and Notch activity in vimentin-negative fibroblasts and epithelial breast cancer cells. (A) The ability of vimentin-negative control SW13 cells or cells transfected with vimentin, Jagged, or vimentin and Jagged to internalize N1ECD–Alexa-488 coupled to protein A agarose beads (N1ECDPrtA). (B) Endocytosis of Jagged in vimentin-negative SW13 cells or in cells transfected with vimentin, Jagged, or vimentin together with Jagged as measured by a fluorescently labeled N1ECD peptide. Values represent means ± SEM. Statistical significance was determined using Student's t test, P < 0.05. Values represent means ± SEM. Statistical significance was determined using Student’s t test, P < 0.05. MFI, mean fluorescence intensity.