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. 2017 May 22;114(23):E4574–E4581. doi: 10.1073/pnas.1703057114

Fig. S4.

Fig. S4.

Vimentin-mediated regulation of Jagged is vimentin phosphorylation dependent. (A) Analysis of Notch signaling potential after pretreatment with the PKC inhibitor bis-indolylmaleimide I (BIM) or the PKC activator Phorbol-12-Myristate-13-Acetate (PMA). The ability of the treated cells to activate Notch in cocultured 293 FLN cells was measured using a luciferase-based reporter system (12xCSL-luc). Values represent means ± SEM. Statistical significance was determined using one-way ANOVA and Bonferroni post hoc test, P < 0.05. (B) Immunoprecipitation of Jagged from vimentin-negative SW13 cells expressing wild-type vimentin, phosphomimicking (VimS4,6,7,8,9D), or phosphodeficient (VimS4,6,7,8,9A) forms of vimentin. (C) Notch activation potential of vimentin-negative control SW13 cells or cells transfected with wild type, phosphomimicking (VimS4,6,7,8,9D), or phosphodeficient (VimS4,6,7,8,9A). Values represent means ± SEM. Statistical significance was determined using Student’s t test, P < 0.05. (D) Receptor-transendocytosis of vimentin-negative control SW13 cells or cells transfected with wild type, phosphomimicking (VimS4,6,7,8,9D), or phosphodeficient (VimS4,6,7,8,9A). Values represent means ± SEM. Statistical significance was determined using Student’s t test, P < 0.05.