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. 2017 May 22;114(23):5994–5999. doi: 10.1073/pnas.1703546114

Fig. 6.

Fig. 6.

Effect of oosporein on fungal virulence, mosquito Duox expression, and midgut bacterial growth. (A) Survival of adult female A. stephensi (n = 50) following topical infection with B. bassiana WT or BbΔops1; control mosquitoes were not infected. (B and C) Effect of Bbops1 disruption on mRNA levels of Duox in the midgut (B) (n = 20) and carcass (C) of mosquitoes (n = 20) following topical infection with B. bassiana WT and BbΔops1. (D) Effect of Bbops1 disruption on midgut total bacteria determined by 16S rRNA gene-based qPCR analysis (n = 15). Experiments were conducted in three biological replicates. Error bars indicate SD. Double asterisks represent a significant difference determined by the Student’s t test at P < 0.01.