Table S1.
Primer | Sequence, 5′-3′ | Application |
16S-27F | AGAGTTTGATCCTGGCTCAG | PCR of eubacterial 16S rRNA gene |
16S-1492R | TACGGYTACCTTGTTACGACTT | |
16SF | TCCTACGGGAGGCAGCAGT | qPCR of eubacterial 16S rRNA gene |
16SR | GGACTACCAGGGTATCTAATCCTGTT | |
SmF1 | ACGTTCATCAATTGACGTTACTCGCA | qPCR of S. marcescens16S rRNA gene |
SmR1 | AACCGCCTGCGTGCGCTTTA | |
338F | ACTCCTACGGGAGGCAGCA | PCR of V3 and V4 hypervariable regions of eubacterial 16S rRNA gene |
806R | GGACTACHVGGGTWTCTAAT | |
cDNA S7F | AGAACCAGCAGACCACCATC | qPCR of AsS7 as endogenous control for quantification of relative expression of immune genes |
cDNA S7R | CGACGCACAGTAGCACAAAC | |
gDNA AsS7F | TCCTGGAGCTGGAAATGAAC | qPCR of AsS7 as endogenous control for quantification of midgut bacteria |
gDNA AsS7R | GCCGGGTCTGAACCTTCTGG | |
GAM1F | GTACGTCAGCCGGAAGGGAG | qPCR of Gambicin 1 |
GAM1R | CGTAATGAACGAGGACGAACAGC | |
CEC1F | GGAAGCGGGACGCCTGAA | qPCR of Cecropin 1 |
CEC1R | CCTTGACACCTGCCACCACC | |
DEF1F | AGTCGTGGTCCTGGCGGCTCT | qPCR of Defensin 1 |
DEF1R | ACGAGCGATGCAATGCGCGGCA | |
ATTF | AAAGCCAGAGCGGCAACAC | qPCR of Attacin |
ATTR | TCAGTAACCGTGCGTGAAAGTC | |
FBN9F | AACAATCTGACCGCACTGC | qPCR of Fbn9 |
FBN9R | TGTGACGCATTCCCTGTAG | |
DuoxF | GGAACTGTTCTCGGCTGTCA | qPCR of Duox |
DuoxR | TGATGTCCCAGAGCGTGAAC | |
gpdF | CCCAGAACATCATTCCCAGC | qPCR of fungal gpd for quantification of B. bassiana |
gpdR | TCAATGCGGGCAGTCAAGTC | |
dsDuoxF | TAATACGACTCACTATAGGGGCTCGTCGGAAGTTTGTGAAAAA | RNAi of mosquito Duox |
dsDuoxR | TAATACGACTCACTATAGGGTCGCATCATCTCACTCAGCTCTCC | |
dsGFPF | TAATACGACTCACTATAGGGAGATGGTGAGCAAGGGCGAGGAGCTGT | RNAi of GFP as negative control |
dsGFPR | TAATACGACTCACTATAGGGAGTTACTTGTACAGCTCGTCCATGCCG |
F, forward; R, reverse.