Table S1.
Primers used in this study
| Primer | Sequence, 5′-3′ | Application |
| 16S-27F | AGAGTTTGATCCTGGCTCAG | PCR of eubacterial 16S rRNA gene |
| 16S-1492R | TACGGYTACCTTGTTACGACTT | |
| 16SF | TCCTACGGGAGGCAGCAGT | qPCR of eubacterial 16S rRNA gene |
| 16SR | GGACTACCAGGGTATCTAATCCTGTT | |
| SmF1 | ACGTTCATCAATTGACGTTACTCGCA | qPCR of S. marcescens16S rRNA gene |
| SmR1 | AACCGCCTGCGTGCGCTTTA | |
| 338F | ACTCCTACGGGAGGCAGCA | PCR of V3 and V4 hypervariable regions of eubacterial 16S rRNA gene |
| 806R | GGACTACHVGGGTWTCTAAT | |
| cDNA S7F | AGAACCAGCAGACCACCATC | qPCR of AsS7 as endogenous control for quantification of relative expression of immune genes |
| cDNA S7R | CGACGCACAGTAGCACAAAC | |
| gDNA AsS7F | TCCTGGAGCTGGAAATGAAC | qPCR of AsS7 as endogenous control for quantification of midgut bacteria |
| gDNA AsS7R | GCCGGGTCTGAACCTTCTGG | |
| GAM1F | GTACGTCAGCCGGAAGGGAG | qPCR of Gambicin 1 |
| GAM1R | CGTAATGAACGAGGACGAACAGC | |
| CEC1F | GGAAGCGGGACGCCTGAA | qPCR of Cecropin 1 |
| CEC1R | CCTTGACACCTGCCACCACC | |
| DEF1F | AGTCGTGGTCCTGGCGGCTCT | qPCR of Defensin 1 |
| DEF1R | ACGAGCGATGCAATGCGCGGCA | |
| ATTF | AAAGCCAGAGCGGCAACAC | qPCR of Attacin |
| ATTR | TCAGTAACCGTGCGTGAAAGTC | |
| FBN9F | AACAATCTGACCGCACTGC | qPCR of Fbn9 |
| FBN9R | TGTGACGCATTCCCTGTAG | |
| DuoxF | GGAACTGTTCTCGGCTGTCA | qPCR of Duox |
| DuoxR | TGATGTCCCAGAGCGTGAAC | |
| gpdF | CCCAGAACATCATTCCCAGC | qPCR of fungal gpd for quantification of B. bassiana |
| gpdR | TCAATGCGGGCAGTCAAGTC | |
| dsDuoxF | TAATACGACTCACTATAGGGGCTCGTCGGAAGTTTGTGAAAAA | RNAi of mosquito Duox |
| dsDuoxR | TAATACGACTCACTATAGGGTCGCATCATCTCACTCAGCTCTCC | |
| dsGFPF | TAATACGACTCACTATAGGGAGATGGTGAGCAAGGGCGAGGAGCTGT | RNAi of GFP as negative control |
| dsGFPR | TAATACGACTCACTATAGGGAGTTACTTGTACAGCTCGTCCATGCCG |
F, forward; R, reverse.