Fig. S5.

BMP signaling is not required for GRN of the DH after stage 23. Hematopoietic programming in the DLP, at stage 26, following heat shock-induced noggin expression at stage 23. The expression of hemangioblast genes are unaffected in the DLP. The expression of hhex gene is absent in the liver primordium (red arrow) in HS:Noggin siblings compared with wild-type siblings (green arrow). Embryos were heat-shocked for 15 min at 35 °C at the stage 23 and were collected for analysis at stage 26. WISH was used to stain cells expressing genes that are essential for hematopoietic programming in the DLP; fli1, gata2, kdr, etv2, tal1, and lmo2 together with noggin transgene transcripts. HS:Noggin transgenic siblings were identified by the expression of Noggin transcripts (Uniform Fast Red staining in HS:Noggin embryos).