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. 2017 May 22;114(23):6005–6009. doi: 10.1073/pnas.1705586114

Fig. 1.

Fig. 1.

(A) Structures of 4CNI, 4CNI-3AA, 4CN-Trp, and 4CN-Trp*-Gly, as indicated. (B) Absorption spectra of Trp, 4CNI, 4CNI-3AA, 4CN-Trp, 4CN-Trp*-Gly, and Gly-Gly-4CN-Trp, as indicated. (C) Comparison of the fluorescence spectrum of indole with those of 4CNI and 4CNI-3AA. These spectra were obtained with an excitation wavelength of 270 nm at 25 °C and the optical densities of all of the samples at 270 nm were equal. (D) Comparison of the fluorescence spectrum of DPA with those of 4CNI, 4CNI-3AA, 4CN-Trp, and 4CN-Trp*-Gly. These spectra were obtained with an excitation wavelength of 325 nm at 25 °C, and the optical densities of all of the samples at 325 nm were equal.