. 2017 May 22;114(23):6005–6009. doi: 10.1073/pnas.1705586114

Table 1.

Fluorescence lifetime (τ) and QYs measured for 4CNI, 4CN-Trp, and their derivatives under different excitation wavelengths, temperatures (T), and solution conditions

Sample	Solvent	T (°C)	τ (ns) (%Amp)	QY 270 nm	QY 325 nm	QY 355 nm
4CNI	H₂O	25	9.1 (100)	0.85 ± 0.13	0.78 ± 0.03	0.64 ± 0.07
4CNI-3AA	H₂O	25	13.5 (100)	0.71 ± 0.04	0.83 ± 0.05^†	0.91 ± 0.14
4CN-Trp*-Gly	H₂O	25	12.6 (100)	0.73 ± 0.10	0.84 ± 0.09	0.82 ± 0.05
4CN-Trp*-Gly	THF	25	7.6 (100)	—	0.62 ± 0.01	0.56 ± 0.06
4CN-Trp*-Gly	Buffer^‡	37	—	—	0.64 ± 0.05	0.74 ± 0.03
4CN-Trp*-Met	H₂O	25	12.2 (100)	—	0.75^§	—
4CN-Trp	H₂O	25	13.7 (95)	—	0.88 ± 0.06^†	—
			1.9 (5)	—		—
Gly-Gly-4CN-Trp	H₂O	25	13.6 (93)	—	0.89 ± 0.05^†	—
			1.7 (7)

^{^†}

Measurements made using the gradient method as described in SI Materials and Methods.

^{^‡}

20 mM phosphate buffer, pH 7.2.

^{^§}

QY determined relative to 4CN-Trp*-Gly.