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. 2017 May 22;114(23):E4648–E4657. doi: 10.1073/pnas.1704698114

Fig. S2.

Fig. S2.

Identification of soluble αSyn assemblies present in human brain tissues. (A) Western blot (WB) profile of soluble αSyn molecules detected in SEC fractions from brain tissue of subjects with normal (Upper) or high (Lower) levels of soluble αSyn. Blue arrows indicate the elution of used globular standards. Samples of interest were separated onto two separate 12-well gels in parallel. (B) Averaged signal intensity for the indicated apparent αSyn assembly detected in each SEC fraction obtained from both subgroups (the solid line represents the mean and shaded areas correspond to SDs). Abnormal elevations were detected for putative αSyn dimers (28 kDa) and monomers (17 kDa) in the brain tissues of AD-high subjects (Kruskal–Wallis followed by Mann–Whitney U test, ★P < 0.05, n = 3 per SEC fraction; n = 5 per group). A.U., arbitrary units.